On September 19, 2025, Prof. Huang Shaohui from the Chinese Academy of Sciences was invited to exchange on fluorescence correlation spectroscopy (FCS) technology and its applications at the Institute of New Concept Sensors and Molecular Materials at Shaanxi Normal University, and presented a report titled “Principles of Single-Molecule Fluorescence Technology in Fluorescence Correlation Spectroscopy and Its Applications in Molecular Interaction Nanoparticle Characterization and Liquid-Liquid Phase Separation”.

Fluorescence Correlation Spectroscopy (FCS) was pioneered in the 1970s by Prof. Watt W. Webb, a member of both the National Academy of Sciences and the National Academy of Engineering in the U.S., along with his colleagues. After more than half a century of development, this technology has been widely applied in research fields such as biology, medicine, chemistry, materials science, optical physics, and micro/nano science. The FCS module developed by Prof. Huang’s team achieves deep integration with Olympus microscopes, overcoming spatial resolution limitations. Within minute detection volumes (corresponding to confocal spots of approximately 1 μm), it performs correlation analysis of fluorescence signal fluctuations over time. This enables quantitative assessment of molecular concentration, fluorescence intensity/oligomerization state, diffusion coefficient/ particle size, interactions, and other properties. The prototype of this technology is now installed at the institute’s Sensing and Materials R&D, Testing, and Evaluation Platform and is available for testing.

The report was chaired by Assoc. Prof. Liu Taihong, with over 40 faculty members and students from the institute in attendance.